Please!
As is, indolebutyric acid (IBA; 2.46 mM), in mg is = (.....)
- As is kinetin (KT; 2.32mM), in mg, is = (.....)
-L-asparagine (Asn)/L-glutamine (Gln) (7.6/13.6 mM) in mg, is = (....)
Thanks amigos. Gracias.
Summary
A new protocol has been developed for the highly efficient somatic embryogenesis and plant regeneration of 10
recalcitrant Chinese cotton cultivars. Calluses and embryogenic calluses were induced on MSB1 medium containing the
optimal combination of indolebutyric acid (IBA; 2.46 mM) and kinetin (KT; 2.32mM). Up to 86.7% of embryogenic
calluses differentiated into globular somatic embryos 2 mo. after culture on MSB2 medium containing double KNO3 and
free of growth regulators. Up to 38.3% of the somatic embryos were converted into complete plants in 8 wk on MSB3
medium with L-asparagine (Asn)/L-glutamine (Gln) (7.6/13.6 mM). The plants were successfully transferred to soil and
grew to maturity. With the protocol described here, we have obtained hundreds of regenerating plantlets from 10
recalcitrant cultivars, which is important for the application of tissue culture to cotton breeding and biotechnology.
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